High Mannose-binding Lectin with Preference for the Cluster of Α1-2 Mannose from the Green Alga Boodlea Coacta Is a Potent Entry Inhibitor of Hiv-1 and Influenza Viruses
نویسندگان
چکیده
HIGH MANNOSE-BINDING LECTIN WITH PREFERENCE FOR THE CLUSTER OF α1-2 MANNOSE FROM THE GREEN ALGA BOODLEA COACTA IS A POTENT ENTRY INHIBITOR OF HIV-1 AND INFLUENZA VIRUSES Yuichiro Sato *, Makoto Hirayama*, Kinjiro Morimoto, Naoki Yamamoto, Satomi Okuyama and Kanji Hori From the Faculty of Pharmacy, Yasuda Women’s University, 6-13-1 Yasuhigashi, Asaminami, Hiroshima 731-0153, Japan, the Graduate School of Biosphere Science, Hiroshima University, Kagamiyama 1-4-4, Higashi-Hiroshima 739-8528, Japan, and the Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2,Blk MD4A, Level 5, Singapore 117597 *Two authors contributed equally to the present study Running title: Antiviral activity of high mannose-binding green algal lectin Author to whom correspondence should be addressed: Dr. Kanji Hori, Graduate School of Biosphere Science, Hiroshima University, Kagamiyama 1-4-4, Higashi-Hiroshima 739-8528, Japan, Phone: +81 82 424 7931; Fax: +81 82 424 7931; E-mail: [email protected]
منابع مشابه
High mannose-binding lectin with preference for the cluster of alpha1-2-mannose from the green alga Boodlea coacta is a potent entry inhibitor of HIV-1 and influenza viruses.
The complete amino acid sequence of a lectin from the green alga Boodlea coacta (BCA), which was determined by a combination of Edman degradation of its peptide fragments and cDNA cloning, revealed the following: 1) B. coacta used a noncanonical genetic code (where TAA and TAG codons encode glutamine rather than a translation termination), and 2) BCA consisted of three internal tandem-repeated ...
متن کاملP 144: Sunflower Mannose binding Lectin-Associated Serine Protease Inhibitor-1 (SFMI-1) and -2: Significant Inhibitors of Mannose binding Lectin Pathway which Helps in Multiple Sclerosis Treatment
One of the important parts of innate immunity is complement system that occurs in three different ways; the classic, the alternative and the lectin pathway. The four pattern recognition molecules that have been identified till now are Mannose binding lectin (MBL), a component of lectin pathway, and three ficolins (ficolin1,-2 and -3) which compound to the carbohydrates of the cell surface. MBL ...
متن کاملA Novel High-Mannose Specific Lectin from the Green Alga Halimeda renschii Exhibits a Potent Anti-Influenza Virus Activity through High-Affinity Binding to the Viral Hemagglutinin
We have isolated a novel lectin, named HRL40 from the green alga Halimeda renschii. In hemagglutination-inhibition test and oligosaccharide-binding experiment with 29 pyridylaminated oligosaccharides, HRL40 exhibited a strict binding specificity for high-mannose N-glycans having an exposed (α1-3) mannose residue in the D2 arm of branched mannosides, and did not have an affinity for monosacchari...
متن کاملEntry Inhibition of Influenza Viruses with High Mannose Binding Lectin ESA-2 from the Red Alga Eucheuma serra through the Recognition of Viral Hemagglutinin
Lectin sensitivity of the recent pandemic influenza A virus (H1N1-2009) was screened for 12 lectins with various carbohydrate specificity by a neutral red dye uptake assay with MDCK cells. Among them, a high mannose (HM)-binding anti-HIV lectin, ESA-2 from the red alga Eucheuma serra, showed the highest inhibition against infection with an EC50 of 12.4 nM. Moreover, ESA-2 exhibited a wide range...
متن کاملHigh Mannose-Binding Antiviral Lectin PFL from Pseudomonas fluorescens Pf0-1 Promotes Cell Death of Gastric Cancer Cell MKN28 via Interaction with α2-Integrin
Novel anti-HIV lectin family which shows a strict binding specificity for high mannose glycans has been found in lower organisms. The bacterial orthologue has been identified in the genome of Pseudomonas fluorescens Pf0-1 and the gene coding a putative lectin was cloned, expressed in Escherichia coli and purified by one step gel filtration. Glycan array screening of the recombinant lectin, term...
متن کامل